Título: Molecular Detection of blaTEM and blaSHV Genes in ESBL-Producing Acinetobacter baumannii Isolated from Antarctic Soil

Autor(es): CABRERA GARCIA JAIRO SANTIAGO, OÑA ROCHA TANIA ELIZABETH, PAZOS MORILLO CLARA ALEXANDRA, PORTILLA CAICEDO KAREN MARLENE, VELARDE CRUZ DELIA ELIZABETH, BANCHON BANCHÓN, CARLOS BASTIDAS-CALDES, FERNANDA HERNANDEZ-ALOMIA, GABRIELA DÁVILA, GUALOTO OÑATE MIGUEL ÄNGEL

Fecha de publicación: 21-feb-2025

Resumen: The phenomenon of antimicrobial resistance (AMR) in cold environments, exemplified by the Antarctic, calls into question the assumption that pristine ecosystems lack clinically significant resistance genes. This study examines the molecular basis of AMR in Acinetobacter spp. Isolated from Antarctic soil, focusing on the blaTEM and blaSHV genes associated with extended-spectrum beta-lactamase (ESBL) production; Soil samples were collected and processed to isolate Antarctic soil bacteria. Molecular detection was then conducted using polymerase chain reaction (PCR) to identify the bacteria species by 16S rRNA/rpoB and 10 different beta-lactamase-producing genes. PCR amplicons were sequenced to confirm gene identity and analyze genetic variability. Acinetobacter baumannii were identified by both microbiological and molecular tests. Notably, both the blaTEM and blaSHV genes encoding the enzymes responsible for resistance to penicillins and cephalosporins were identified, indicating the presence of resistance determinants in bacteria from extreme cold ecosystems.

Palabras clave: antibiotic resistance genes; cold environment; human-impacted environment

DOI: https://doi.org/10.3390/microorganisms13030482

ISSN: 2076-2607

Tipo publicación: Artículo